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Development of MS-based analytical methods for glycomics & glycoproteomics

Information on composition and structure of glycans, glycan heterogeneity, and glycosite occupancy is increasingly recognized as crucial to understanding glycan and glycoprotein structure and function in a biological system. Yet, no sensitive approach currently exists for the analysis of glycome and glycoproteome despite a biological importance of glycosylation. The AGRS has developed new and improved analytical techniques for comprehensive glycan and glycosite profiling using high performance mass spectrometry that can be applied in glycoproteomics and glycomic research.

Research highlights:
  • Development of analytical platform for glycomic characterization of bioactive glycans on biotherapeutics using stepwise PGC SPE and LC-MS/MS
  • Cell surface glycome (glycan, glycolipid) mapping
  • Absolute glycan quantitation using label-free LC/MRM-MS
  • Efficient and selective method to capture O-glycans from complex mixture
  • Isomer-specific glycan separation and quantitation
  • Selective enrichment of phosphoglycans using newly designed LC/MS chip
Relevant publications:
  • Kim, J., Yin, D., Lee, J., An, H. J., & Kim, T. Y., "Deuterium Oxide Labeling for Global Omics Relative Quantification (DOLGOReQ): Application to Glycomics.", Analytical Chemistry, 2021.
  • Oh, M. J., Seo, N., Seo, J., Kim, G. H., & An, H. J., "Facile and Rapid Glycosylation Monitoring of Therapeutic Antibodies Through Intact Protein Analysis.", Mass Spectrometry Letters, 2021, 12(3), 85-92.
  • Seo, N., Ko, J., Lee, D., Jeong, H., Oh, M. J., Kim, U., Lee, D. H., Kim, J., Choi, Y. J., An, H. J., “ In-depth characterization of non-human sialic acid (Neu5Gc) in human serum using label-free ZIC-HILIC/MRM-MS”, Analytical and bioanalytical chemistry, Analytical and bioanalytical chemistry, 2021, 413(20), 5227-5237.
  • Lee, J., Hwang, H., Kim, S., Hwang, J., Yoon, J., Yin, D., Choi, S. I., Kim, Y. H., Kim Y. S., An, H. J., "Comprehensive profiling of surface gangliosides extracted from various cell lines by LC-MS/MS.", Cells, 2019, 8(11), 1323.
  • Seo, Y., Oh, M. J., Park, J. Y., Ko, J. K., Kim, J. Y., An, H. J., "Comprehensive characterization of biotherapeutics by selective capturing of highly acidic glycans using stepwise PGC-SPE and LC/MS/MS.", Analytical chemistry, 2019, 91(9), 6064-6071.
  • Lee, H. H. L., Heo, C. E., Seo, N., Yun, S. G., An, H. J., & Kim, H. I., "Accurate quantification of N-glycolylneuraminic acid in therapeutic proteins using supramolecular mass spectrometry.", Journal of the American Chemical Society, 2018, 140(48), 16528-16534.